Making a pH Buffer Solution
"A buffer solution is water mixed with a chemical to give it special properties in regards to
pH (acidity). The chemical, known as a buffer agent, resists pH changes when exposed to acids
and bases when properly mixed in a solution. This property makes it extremely useful in protecting
sensitive equipment, dealing with chemical accidents, and even in balancing the internal processes
of “living things." A great example of a buffered solution is your blood. It is the combination or
presence of carbonic acid (H2CO3) and bicarbonate (HCO3-) in the blood that keeps the pH of the blood in the proper range (Source).
In the table below, the masses give 0.1 M concentrations of each species, when diluted to 1.0 L,
so that the [acid]:[conjugate base] ratio is unity. In most cases the pH will have to be adjusted
using a pH meter and the dropwise addition of either 6 M HCl or 6 M NaOH to one liter of solution
until the desired pH is reached.
Phosphoric acid 85%
H3PO4
6.8 mL |
Monopotassium phosphate
KH2PO4
12.8 g |
2.1 |
Hydrochloric acid
HCl
8.6 mL |
Potassium hydrogen
phthalate
KHC8H4O4
20.4 g |
2.8 |
Citric acid
HOC(CH2CO2H)2CO2H
19.2 g |
Monopotassium citrate
HOC(CH2CO2H)2CO2K
23.0 g |
3.1 |
Methyl succinic acid
HO2CCH2CH2CO2H
11.8 |
Monosodium methylsuccinate
HO2CCH2CH2CO2Na
14.0 |
4.1 |
Acetic acid
CH3CO2H
glacial
5.7 mL or
Acetic acid
CH3CO2H
dilute (36%)
15.9 mL |
Sodium acetate
CH3CO2Na
8.2 g |
4.75 |
Potassium hydrogen
phthalate
KHC8H4O4
20.4 g |
Sodium hydroxide
NaOH
4.0 g |
4.8 |
Monosodium
methylsuccinate
NaO2CCH2CH(CH3)CO2H
15.3 g |
Disodium methylsuccinate
NaO2CCH2CH(CH3)CO2Na
17.5 g |
5.6 |
Monosodium citrate
HOC(CH2CO2H)2CO2Na
21.4 g |
Disodium citrate
NaOC(CH2CO2H)2CO2Na
23.6 g |
5.9 |
Disodium citrate
HOC(CH2CO2Na)2CO2H
23.6 g |
Trisodium citrate
HOC(CH2CO2Na)2CO2Na
25.8 g |
6.4 |
Monopotassium phosphate
KH2PO4
12.8 g |
Dipotassium phosphate
K2HPO4
15.8 g |
7.2 |
DL-Cysteine
HSCH2CH(NH2)CO2H
12.1 g |
Sodium DL-Cysteinate
HSCH2CH(NH2)CO2Na
14.3 g |
8.1 |
Boric acid
H2B4O7
31.4 g |
Sodium Hydroxide
NaOH
4.0 g |
9.1 |
Sodium bicarbonate
NaHCO3
8.4 g |
Sodium carbonate
Na2CO3
10.6 g |
10.2 |
Monosodium tellurate
NaHTeO4
21.6g |
Disodium tellurate
Na2TeO4
23.8g |
11.3 |
Monosodium ascorbate
NaC6H7O6
19.8g |
Disodium ascorbate
Na2C6H7O6
22.0g |
11.8 |
Dipotassium phosphate
K2HPO4
15.8 g |
Tripotassium phosphate
K3PO4
19.6 g |
12.7 |
Boric acid
H2B4O7
31.4 g |
Sodium hydroxide
NaOH
12.0 g |
12.7 |